(2 - (5 - nitro - 2 - furyl) - vinyl) - pyrimidine derivative and process for the preparation thereof



.Ufl w ms P te ABSTRACT OF THE DISCLOSURE Novel 2-(5 nitro-2-furyl)-vinyl pyrimidine derivatives having the formula wherein R stands for a member selected from the group consisting of alkylthio, amino, alkylarnino, dialkylamino and alkanoylamino, said alkyl and alkanoyl each having 1-4 carbon atoms, and R represents a member selected from a hydrogen atom and an alkyl group havingl-4 carbon atoms.

It has now been found out that a novel-[-2-(5- nitro'-2- furyl)-vinyl] -pyrimidine derivative of the formula NOl0 'CH=CH N (wherein R stands for a substituent selected from the group consisting of alkylthio, amino, alkylamino, dialkylamino and alkanoylamino, said alkyl and alkanoyl having 1-4 carbon atoms, and R represents a hydrogen atom or an alkyl group having 14 carbon atoms) has excellent antibacterial and antiprotozoal activities.

A preferable compound is that which whose Z-(S-nitro- 2-furyl)-vinyl group is at 4-position of a pyrimidine nucleus and whose substituent R is at Z-position of the pyrimidine nucleus, above all, whose substituent R is methylthio, amino or acetylamino.

The new compounds of this invention have high activities against important Gram positive and Gram negative strains of pathogenic bacteria such as Staphylococcus aureus, Shigella flexneri and so forth. Further, the compounds have antitrichomonal activities. It can be expected that the compounds are useful in the treatment of bacterial and protozoal infections in man and animals.

Patented Sept. 2, 1969 The following table summarizes the in vitro activities of the compounds against a variety of microorganisms. The minimum inhibitory concentration (MIC) was determined by Well known serial dilution technique.

TABLE [Antimicrobial activity in vitro (MIC: meg/1131.)]

Compound Organism N.F. 243 N.F. 276 N.F. 450

Staphylococcus aureus 0. 1 0.3 0. 3 Staphylococcus albns. 0. 1 0. 1 0. 3 Diplocaccas pnenmoniae I... 30 0. 3 0. 3 Diplocncc'us pnenmoniae II. 1 0. 1 Diplococcus pneumoniae III. 0. 1 0.03 Escherichia coli 0. 3 0. 1 0. 3 Klebsiella pneumoniae..-. 0.3 0. 1 0. 3 Salmonella paratyphi A.- 1 1 30 Salmonella enteridis 1 O. 3 l Salmonella typhimnrinm. 0.3 O. 3 3 Shz'gella flexneri 2a 1 0.3 1 Shigella sonnei 0. 3 0. 3 1 Bacillus 8ublilia..... 0. 01 O. 01 0. 1 Bacillus megaterinm. 0. 03 -O. 03 0. 1 Listeria monocytogene 0. 3 1 30 Salmonella pulloram 0. 3 0. 1 l Mycobacterium tuberculosis 1 3 10 Trichomonas vaginalz's 0. 3 10 8 NF. 243 2-methylthio-4- [2-(5-nitro-2-furyl) -vinyl] pyrimidin-6-ol.

, NR 276: 2-acetamino-4-[2-(5-nitro-2-furyl)-viny1] pyrimidin-6-ol.

, N.F. 450: 2-amino-4-[2-(5-nitro-2-furyl)-vinyl]-pyrimidin-6-ol.

The novel compound of the aforesaid Formula I can be prepared by condensing S-nitrofurfural or its diacetate with the corresponding substituted methylpyrimidin-fi-ol or 6-alkylether by a process known per se.

This condensation reaction can be advantageously carried out by heating a mixture of S-nitrofurfural (or its diacetate) and the substituted methylpyrimidin-fi-ol or -6-alkylether in an organic inert solvent in the presence of a dehydrating agent, for instance at temperatures from C. to C. As the dehydrating agent, a lower fatty acid anhydride such as aceticarhydride as well as phosphoric acid, hydrochloric acid, sulfuric acid or anhydrous zinc chloride are suitable, and with an alkali condensing agent such as piperazine, the yield is not good. As the solvent of this reaction, either said dehydrating agent is used in a large amount concurrently as a solvent or an inert solvent such as glacial acetic acid, an alcohol and dioxane may be utilized.

When the case of S-nitrofurfural and 2-amino-4-methylpyrimidin-6-ol are taken as an example, this condensation reaction is explained as follows.

NOZUCHO CHa-QN a The compound of this invention whose R is an alkanowith water and ether and recrystallized from acetone, and

yla'mino group can be converted 'to the corresponding A mixture of 8.5 grams of S-nitrofurfural, grams of 2-acetamino-4-methy1pyrimidin-6-ol and milliliters of acetic anhydride was heated at 120-125 C. for 7 hours. After cooling, when the separated crystals were filtered and recrystallized from a mixture of dimethylformamide and water, 10.5 grams of 2-acetamino-4-[2-(5-nitro-2- furyl)-vinyl]-pyrimidine-6-ol were obtained. The melting point was 300 C. (decomposition).

0.9 gram of 2-acetamino-4-[2-(5-nitro-2-furyl)vinyl]- pyrimidin-6-ol and milliliters of hydrochloric acid diluted to 20% by ethanol was heated at C. on a water bath for 2 hours. After cooling, the mixture was neutralized by saturated sodium bicarbonate, the crystals were filtered and washed with water, thereafter when the crystals were recrystallized from dimethylformamide, 0.6 gram of 2-amino-4-[2-(5-nitro-2-furyl)-vinyl]-pyrimidin- 6-01 was obtained. The melting point was 300 C. (decomposition) a Example 2 2.8 grams of S-nitrofurfural and 2.5 grams of Z-amino- 4-methylpyrirnidin-6-ol were dissolved in 20 milliliters of glacial acetic acid containing 2 grams of concentrated sulfuric acid, and the mixture was heated at 80 C. on a water bath for 5 hours. After cooling, the reaction mixture was added with water, neutralized by saturated aqueous sodium bicarbonate, and when the separated crystals were recrystallized from a mixture of dimethylformamide and water, 2 grams of 2-amino-4-[2-(5-nitro-2- furyl)vinyl]pyrimidin-6-ol were obtained. The melting point was 300 C. (decomposition).

Example 3 A mixture of 1.4 grams of S-nitrofurfural, 1.6 grams of 4-methyl-2-methylthiopyrimidin-G-ol and 3.1 grams of acetic anhydride was heated at -130 C. for 6 hours. After cooling, the separated crystals were washed 1' gram of 2-methylthio-4 [Z-(S-nitrO-Z-fuiyl)-vinyl]-py rimidin-6-ol was obtained. The melting point was 278 C.

Example 4 1.41 grams of S-nitrofurfural and 1.70 grams of 4- methoxy-6-methyI-Z-methylthiopyrimidine were dissolved in 3.1 milliliters of acetic anhydride, and the mixture was heated at C. on an oil bath for 9.5 hours. After cooling, the separated crystals were washed with ether and recrystallized from acetone, and 0.9 gram of 6-meth0xy- 2-me'thylthio-4- [2- (S-nitro-Z-furyl) -vinyl] -pyrir nidine was obtained. The melting point was 204-206 C.

What is claimed is: .1; A' 2-substituted-4-[2-( 5-nitro-2-furyl)-viny1]-pyrimidine derivative of the formula References Cited FOREIGN PATENTS 630,163 10/1963 Belgium.

26,408 11/1964 Japan. 14,029 7/1965 Japan. 13,877 7/1965 Japan.

JOHN D. RANDOLPH, Primary Examiner US. Cl. X.R. 260999 UNITED STATES PATENT OFFICE CERTIFICATE OF CORRECTION Patent No. 3,464 ,982 September 2 1969 Shinsaku Minami et a1 It is certified that error appears in the above identified patent and that said Letters Patent are hereby corrected as shown below:

Column 2 lines 50 to 55 the formula should appearzas sh below:

I I H2 [N0 L j CI-IO CH Signed and sealed this 10th day of November 1970 (SEAL) Attest:

WILLIAM E. SCHUYLER, JR.

Edward M. Fletcher, Ir.

Commissioner of Patents Attesting Officer 

